Host cell receptor for Nipah and Hendra viruses identified

first_img See also: NIAID press release As the PNAS article notes, “There are presently no vaccines or approved therapeutics for NiV or HeV infection.” Knowing both the viral and cell proteins involved in fusion will allow scientists to develop vaccines and treatments for these two important emerging infectious diseases. Both groups highlighted the close concordance between patterns of Ephrin-B2 expression and sites of infection. “EphrinB2 is essential for vasculogenesis and axonal guidance, and is expressed on endothelial cells, neurons and smooth muscle cells surrounding small arteries and arterioles—an expression pattern highly concordant with the known cellular tropism of NiV,” the Nature report says. The UCLA group’s findings are detailed in a Jul 6 online publication in Nature. Dr. Lee and colleagues utilized the NiV attachment glycoprotein (G) responsible for host cell fusion in an immunoprecipitation assay. They found that G pulled down a 48kD protein from the surface of susceptible, but not resistant, cells. Using trypsin digestion and mass spectrometry, they identified the protein as Ephrin-B2. Proceedings of the National Academy of Sciences published the USUHS group’s findings online Jul 5. The authors had previously identified a human cell line not susceptible to infection by NiV or HeV. Using microchips embedded with thousands of candidate genes, they compared genes expressed in this resistant cell line to those in cell lines susceptible to viral infection. Twenty-one candidate genes were expressed only in the susceptible cell lines and from this group, the researchers identified the 10 genes expressed most strongly in the susceptible cells. These were transfected individually into the resistant cell line; only Ephrin-B2 caused the resistant cells to become susceptible to infection. The two groups also pointed out the conserved nature of the Ephrin family of cell surface proteins and noted that this could explain the broad range of species susceptible to infection by Nipah and Hendra viruses. Observing that NiV and HeV have been documented in flying foxes, the PNAS article says, “It will be of interest to determine whether [Ephrin-B2] serves as the virus receptor in these natural animal hosts.” CIDRAP Overview on Nipah virus Bonaparte MI, Dimitrov AS, Bossart KN, et al. Ephrin-B2 ligand is a functional receptor for Hendra virus and Nipah virus. Proc Natl Acad Sci 2005 (published online Jul 5) [Full text]center_img Jul 19, 2005 (CIDRAP News) – Two groups have independently published the identity of the human protein that facilitates infection by the Nipah and Hendra viruses. First recognized in the mid-1990s, these two emerging infectious animal diseases, which are transmissible to humans, are considered possible bioterror agents. A Malaysian outbreak of respiratory infections among pigs in 1998 spread to nearly 370 people in the form of a viral encephalitis, killing 105. The identified viral pathogen, named Nipah virus (NiV), was also classified as a paramyxovirus. In 1994, 15 horses and two humans near Brisbane, Australia, presented with fatal acute respiratory disease. The etiologic agent was named Hendra virus (HeV) and classified in a new genus of the viral family Paramyxoviridae. Though the viral proteins responsible for attachment were identified, no one had yet found the receptor that allows Nipah and Hendra viruses to enter host cells. The new studies point to a cell surface protein, Ephrin-B2, as the mechanism for entry. The protein is present in humans, horses, pigs, bats, and other mammals – a finding consistent with the wide range of animals susceptible to Nipah and Hendra infection. A team led by Christopher Broder, PhD, at the Uniformed Services University of the Health Sciences (USUHS) utilized a microarray screen to narrow down the possible receptors. Working independently, Benhur Lee, MD, and colleagues at the University of California–Los Angeles (UCLA) identified the receptor using an immunoprecipitation strategy. Negrete OA, Levroney EL, Aguilar HC, et al. EphrinB2 is the entry receptor for Nipah virus, an emergent deadly paramyxovirus. (Letter) Nature 2005 (published online Jul 6) [Abstract] Both studies were funded by the National Institute of Allergy and Infectious Diseases (NIAID).last_img read more

Airports recorded an increase in traffic

first_imgThe total number of aircraft landings and take-offs at airports in February 2019 was 4, which is an increase of 442% compared to February 2018. The largest passenger traffic was generated by Zagreb Airport with 179 thousand passengers (an increase of 5,8% compared to February 2018), followed by Dubrovnik Airport with 34 thousand passengers (an increase of 39,0% compared to February 2018) and the airport Split with 33 thousand passengers (an increase of 13,7% compared to February 2018). In February 2019, Croatian airports recorded 252 thousand passengers or 9,9% more than in the same month last year, according to data from the Central Bureau of Statistics (CBS). center_img The most significant international passenger traffic was realized with German airports, 72 thousand passengers, which is an increase of 17,9% compared to the same period last year.last_img read more

Laurent Koscielny sends message to Gabriel Paulista and Francis Coquelin ahead of Arsenal reunion

first_imgLaurent Koscielny sends message to Gabriel Paulista and Francis Coquelin ahead of Arsenal reunion Comment Laurent Koscielny has urged Arsenal to take a lead to Valencia (Picture: Getty)Laurent Koscielny has urged Arsenal supporters to give a ‘welcoming reception’ to former Gunners Francis Coquelin and Gabriel Paulista.Paulista joined Valencia in 2017 after two-and-a-half-years at the Emirates, while Coquelin moved to Spain a year later.Coquelin is suspended for tonight’s first leg but has travelled with his team-mates and Gabriel is expected to start at centre-back.Koscielny says both players were popular members of the dressing room and has urged supporters to show their love for the returning pair.ADVERTISEMENT Coquelin is suspended for the first leg but Gabriel is expected to start (Picture: Getty)‘Facing Valencia means that we will play against Gabriel and Francis Coquelin, our former teammates here,’ Koscielny wrote in his notes for the game.AdvertisementAdvertisement‘I expect a welcoming reception from you for them. They are really good guys, really liked by the dressing room when they were at the club and they gave everything they had for Arsenal.‘Francis is suspended tonight but they are both doing well in Spain and we are really happy for them.’More: Arsenal FCArsenal flop Denis Suarez delivers verdict on Thomas Partey and Lucas Torreira movesThomas Partey debut? Ian Wright picks his Arsenal starting XI vs Manchester CityArsene Wenger explains why Mikel Arteta is ‘lucky’ to be managing ArsenalArsenal have reached the Europa League semi-finals for a second consecutive year after their exit to eventual winners Atletico Madrid in 2018.Koscielny tore his Achilles during the tie but says that the defeat can serve as inspiration tonight.‘Certainly the loss against Atletico last season will be useful for us,’ said Koscielny.‘We know what it is like to play at Europa League semi-final. We have this experience in our locker, especially on how to cope with the pressure of being so close to the final and a potential trophy’.MORE: Tottenham announce Jan Vertonghen injury update ahead of Bournemouth clash Advertisement Sean KearnsThursday 2 May 2019 6:38 pmShare this article via facebookShare this article via twitterShare this article via messengerShare this with Share this article via emailShare this article via flipboardCopy link238Shares Advertisementlast_img read more

Arsenal’s Pierre-Emerick Aubameyang heaps praise on two Liverpool players

first_imgArsenal’s Pierre-Emerick Aubameyang heaps praise on two Liverpool players Mane, Salah and Aubameyang at the African Footballer of the Year Awards in 2018 (Picture: Getty Images)‘It meant a lot to win the Golden Boot,’ Aubameyang told Arsenal’s official website. ‘I am really happy and proud of it, especially to share the trophy with Sadio Mane and Mohamed Salah.AdvertisementAdvertisement‘I really like these two guys. We are representing Africa so it is a good sign for the continent. Africa is in my heart and in my mind, so I’m extremely proud to represent it in the Premier League.‘The African people really love football, like everyone across the world, it makes me very proud to represent Africa in such an amazing way because not everyone gets the chance to play in the Arsenal colours.’More: FootballRio Ferdinand urges Ole Gunnar Solskjaer to drop Manchester United starChelsea defender Fikayo Tomori reveals why he made U-turn over transfer deadline day moveMikel Arteta rates Thomas Partey’s chances of making his Arsenal debut vs Man CityAfter an impressive season in front of goal for the Gunners, Aubameyang is set to be offered a new contract, as is strike partner Alexandre Lacazette.The Gabonese forward has been linked with a move to China, while Lacazette has been mentioned as a Barcelona target.Arsenal wish to ward off interest in their two key strikers by extending their deals at the club.MORE: No Liverpool transfer offer for Nabil Fekir, insists Lyon presidentMORE: PSG president rubbishes rumours of Kylian Mbappe moving to Liverpool Phil HaighWednesday 19 Jun 2019 5:22 pmShare this article via facebookShare this article via twitterShare this article via messengerShare this with Share this article via emailShare this article via flipboardCopy link100Shares Pierre-Emerick Aubameyang has high praise for two Liverpool stars (Picture: AMA/Getty Images)Arsenal striker Pierre Emerick-Aubameyang is a big fan of the two men he shared the Premier League Golden Boot with, Liverpool’s Mohamed Salah and Sadio Mane.All three men scored 22 league goals this season and shared the prize as a result, the first time it has been split three ways since 1998-99 when Michael Owen, Dwight Yorke and Jimmy-Floyd Hasselbaink finished level.Winning the award for the first time was a proud moment for Aubameyang and sharing it was not an issue, especially as it was with his fellow African stars Salah and Mane.Aubameyang of Gabon, Mane of Senegal and Salah of Egypt are three of the biggest stars in the Premier League, and the Arsenal man is delighted that they are all representing their continent with distinction.ADVERTISEMENT Advertisement Comment Advertisementlast_img read more

GO-TIME: Commonwealth Receives National, Statewide Recognition for Public Works Modernization, Process Improvement Efforts

first_img Efficiency,  GO-TIME,  Government That Works,  Innovation,  Press Release Harrisburg, PA –  Pennsylvania Department of General Services Secretary Curt Topper today announced that the department’s Public Works Modernization initiative recently received national recognition by earning e-Builder’s 2017 “Innovators in Construction” award for the public sector and statewide honors by receiving the highly competitive Governor’s Office of Transformation, Innovation, Management and Efficiency, or GO-TIME, Award.“The Public Works Modernization effort is a prime example of how we deliver a Government that Works for all Pennsylvanians,” Governor Tom Wolf said. “I commend the DGS team for embracing the challenge of working smarter, implementing new technology and delivering greater value for our citizens, a better experience for our contractors and a faster solution for our agencies’ construction needs.”Through a partnership with e-Builder, the Department of General Services streamlined the way it manages building projects for state agencies, integrated all processes from capital planning, design, electronic bidding, construction and project closeout.  Along with these integrations, anyone from senior management to contractors working on a job site, are able to access a multitude of reports and create customizable dashboards.  This implementation also resulted in Public Works moving from a paper world, to being virtually paperless. The department is currently managing 183 design and 80 capitol construction projects in the new system.“The recognition being received for our Public Works Modernization efforts is a testament to the hard work, dedication and commitment exhibited by everyone involved in this initiative,” Secretary Topper said. “While the awards may read “Department of General Services” on them, the real winners are our customers who will be the beneficiaries of a streamlined construction process from start to finish.”The department also used the modernization initiative as an opportunity identify inefficient processes, streamline them and then use technology to implement changes. Under the previous paper-laden process, it took five days to input bids for a single project. Today, the same task can be completed in a matter of minutes.Secretary Topper also noted that it used to take the department an average of 79 calendar days to identify, authorize and approve a single change order. Thanks to a process improvement that gives field staff the ability and authority to handle change orders up to a certain amount on the worksite, combined with the e-Builder software solution, the time is down to an average of seven days.  “In fact, the first change order to go through the process using the e-Builder technology was completed in about five hours!” Topper added.The e-Builder Innovators in Construction award recognizes organizations that demonstrate excellence in using technology to drive substantial building project budgets and schedule savings. See the project award video here.The GO-TIME Awards are presented annually to recognize initiatives that are improving state government operations through cost-savings to taxpayers, efficiency gains for employees and better customer service to the public.To learn more about GO-TIME, visit GO-TIME: Commonwealth Receives National, Statewide Recognition for Public Works Modernization, Process Improvement Efforts October 18, 2017center_img SHARE Email Facebook Twitterlast_img read more

Angola LNG cancels tender due to production glitch

first_imgLNG World News Staff Image courtesy of Angola LNGChevron-led Angola LNG project has reportedly moved to cancel a tender to sell a spot cargo following production issues. Traders were informed that the tender has been withdrawn due to a ‘minor problem at the Soyo plant’ which affected production, Reuters reports, citing market sources.The tender was issued last week and the cargo was scheduled for loading on January 10-11.Angola LNG is a joint venture between Chevron (36.4%), Sonangol (22.8%), BP (13.6%), Eni (13.6%), and Total (13.6%) and has the capacity to produce 5.2 million tons per year at its facility in Soyo.last_img read more

Top news of the week June 24-29

first_imgLNG World News Staff Image courtesy of MOLLNG Limited updates Magnolia LNG contract validityPerth-based LNG Limited has agreed to an updated legally binding lump sum turnkey EPC contract with the KBR-SKE&C joint venture (KSJV) for the Magnolia LNG project.Samsung C&T to build Vietnam’s first LNG terminalThe consortium led by the South Korean Samsung C&T, a Samsung Group company, has won a deal to build Vietnam’s first liquefied natural gas (LNG) terminal.Wood Mackenzie: Mozambique LNG one of top three sanctions this yearAnadarko’s Mozambique LNG project is deemed as one of the top three most valuable oil and gas sanctions taken this year, according to consultancy group Wood Mackenzie.Hong Kong LNG Terminal charters MOL’s FSRU ChallengerJapan’s shipping giant Mitsui O.S.K. Lines (MOL) has entered into a long-term charter deal with Hong Kong LNG Terminal for a floating storage and regasification unit (FSRU).Twelve companies express interest in Bangladesh LNG projectTwelve companies have expressed interest in building Bangladesh’s first onshore liquefied natural gas (LNG) import terminal.last_img read more

Bulldogs Swimming Team Gets 400th Win

first_imgBulldogs record 400th win!The boys recorded the 400th win for Batesville with their wins against LHS and Milan. The boys overall record is 400-193-2 since the 2002-2003 inaugural season. The girls recorded the 300th win for BHS earlier this season and are 307-296-1 after tonight’s two wins.Boys Team Results: BHS 121, Milan 89, LHS 62.Boys are 17-10 on the season (8-5 in EIAC, 9-5 non-conference).Individual Event Winners: Miller 50 Free/100 Free, K Main 100 FlyGirls Team Results: BHS 153, LHS 63, Milan 60.Girls are 25-6 on the season (10-4 in EIAC, 15-2 non-conference)Individual Event Winners: Daulton 200 Free, Gutzwiller 200 IM/100 Breast, M Poltrack 100 Fly, Villani 100 Free, El Weiler 500 Free.Courtesy of Bulldogs Coach T. J. Greene.last_img read more

Eagles And Squires Battle It Out In Cross Country

first_imgThe Jac-Cen-Del Junior High Cross Country teams ran in a dual meet with South Dearborn at Moores Hill Elementary last night (8/28). The JCD Girls’ team finished 2nd and the JCD Boys’ team finished 1st in the meet.Aug28@Moores Hill – South Dearborn (1)Courtesy of Eagles Coach Steve Narwold.last_img

Abelson adds third Tiny Lund trophy to collection

first_imgHARLAN, Iowa (Aug. 29) – For the third time in his career, Chris Abelson stood tallest at Shelby County Speedway’s Tiny Lund Memorial.Abelson held off Nick Deal in winning Saturday’s 25-lap main event for Xtreme Motor Sports IMCA Modifieds.“It was definitely a battle,” the national point leader said following his 31st victory of the season. “It was a good race and fun for sure.”Abelson drew the inside row four start and was in the lead when the fifth circuit was scored. He’d trade the lead with Sobbing and led to the finish once he got back in front.The late-race storyline included lapped traffic and one final restart. Deal was threatening on the high side, so Abelson moved higher on the oval himself.Deal, Sobbing, Jacob Anson and Adam Larson completed the top five. Along with the $1,000 checkers, Abelson earned a spot on the ballot for the 2016 Fast Shafts All-Star Invitational.He’d also won the Tiny Lund main event for Karl Chevrolet Northern SportMods in 2006 and the Modified headliner in 2010.David Livingston also pocketed $1,000 by winning the IMCA Late Model feature. The victory was his first of the season and Tiny Lund career first.“I’ve been racing a Late Model for 16 years and every year they’ve had Late Models at the Tiny Lund, I’ve been trying to win here,” the Glenn Robey Motorsports driver said. ”This was a great way to end the season.”Livingston was in front coming out of the first set of turns. Cooney took charge following a restart with 11 laps left and put some distance on the field before Livingston began making up ground.The two were side-by-side at the white flag. Livingston proved to have the faster line inside, taking his Butch Lavell, Keith Manders, Alpine Inn-backed ride across the stripe just ahead of Cooney.Robert See, Barry Sorensen and Kyle Segebart were scored next.Brian Blessington was the IMCA Sunoco Stock Car winner, ahead of 16th starting Daniel Hilsabeck and 11th starting Brad Derry.Top Northern SportMod honors went to Cody Olsen. Tenth starting Clint Luellen was the runner-up and Glenn Gladson Jr. ran third.Corey Madden captured the IMCA Sunoco Hobby Stock checkers ahead of Jeff Anderson and Shannon Anderson.Alec Stapleton won the Mach-1 Sport Compact main. Taylor Griffin raced all the way from 18th to second; Tyler Thompson started 11th and finished third.Feature ResultsModifieds – 1. Chris Abelson; 2. Nick Deal; 3. Jesse Sobbing; 4. Jacob Anson; 5. Adam Larson; 6. Brandon Monson; 7. Travis Hatcher; 8. Craig Reetz; 9. Robert Tippery; 10. Tom Berry Jr.; 11. John Davis; 12. Ricky Stephan; 13. Cody Hillgartner; 14. Ryan Topf; 15. Nate Caruth; 16. Brian Foote; 17. Jared Hansen; 18. Jonathan Plowman; 19. Mike Albertsen; 20. Steve Reetz; 21. Jeff Stephens; 22. Jake Neal; 23. Andy Miller; 24. Jacob Murray; 25. Dustin Smith.Late Models – 1. David Livingston; 2. Todd Cooney; 3. Robert See; 4. Barry Sorensen; 5. Kyle Segebart; 6. Vic Lovejoy; 7. Daulton Maassen; 8. Bill Hoover; 9. Don Beckstrom; 10. Jeff Segebart; 11. Paul Nagle; 12. Allan Hopp; 13. Mark Plowman; 14. Leon Zeitner.Stock Cars – 1. Brian Blessington; 2. Daniel Hilsabeck; 3. Brad Derry; 4. Keith Knop; 5. Joe Zadina; 6. Mike Stapleton; 7. Jay Brannan; 8. Adam Hensel; 9. Tanner Powers; 10. J.J. Baumli; 11. Tony Scheffler; 12. Chris Ullrich; 13. Joe Smith; 14. Derrick Rohe; 15. Bryan Snell; 16. Ron Bougher; 17. Ray Dessel; 18. Justin Bromert; 19. Dale Woodard; 20. Bob Walker; 21. Mike Harris; 22. Clay Mercer; 23. Trent Partridge; 24. Devin Smith.Northern SportMods – 1. Cody Olsen; 2. Clint Luellen; 3. Glenn Gladson Jr.; 4. Keagan Scott; 5. Kody Havens; 6. Shawn Harker; 7. Kaleb Kennedy; 8. Jake Sachau; 9. Thomas Van Dyke; 10. D.J. Doebelin; 11. Ed Hamilton; 12. Matt Richards; 13. Kevin Bruck; 14. Shawn Cooney; 15. Rodney Slechta; 16. Dan Kline; 17. Tim Judd; 18. Randy Archer; 19. Brett Hopp; 20. Ron Tex; 21. Evan Nemitz; 22. Galen Grabill; 23. Mike Brown; 24. Dylan Petersen; 25. Logan Scott.Hobby Stocks – 1. Corey Madden; 2. Jeff Anderson; 3. Shannon Anderson; 4. Jason Fusselman; 5. Jeff Fink; 6. Andy Davison; 7. Tom Myers; 8. Daniel Smith; 9. Chuck Madden Jr.; 10. Josh Cook; 11. Colby Christensen; 12. Willie Ransom; 13. Kevin Bruck; 14. Randy Anderson; 15. Jason Kohl; 16. Mike Pruitt; 17. Jesse Totten; 18. Sterling Sorensen; 19. Jordan Androy.Sport Compacts – 1. Alec Stapleton; 2. Taylor Griffin; 3. Tyler Thompson; 4. Bronson Griffin; 5. Jeff Stephens Jr.; 6. Nolan Parker; 7. David Sciranko; 8. Thomas Kurtz; 9. Terry Romick III; 10. Deb Braasch; 11. Corey Stapleton; 12. Adam Rehse; 13. Cody Thompson; 14. Jesse Totten; 15. Jason Kroeger; 16. Randy Rush; 17. Alex Davis; 18. Tony Kerger; 19. Tim Hoffman.last_img read more